解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A new series of oxy-peptide nucleic acids (pyrrolidine-based oxy-peptide nucleic acids = POPNAs) of four different stereoisomeric forms (cis-L, cis-D, trans-L, trans-D) have been synthesized. To find a favorable stereoisomer of POPNA for hybridization with DNA or RNA, thermodynamic parameters and conformations of the ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20364
更新日期:2006-01-01 00:00:00
abstract::Because of its involvement in HIV entry, the chemokine receptor CXCR4 is an attractive target for antiretroviral drugs. Despite the large number of CXCR4 inhibitors studied, the 3D pharmacophore for binding to CXCR4 remains elusive, mainly as a result of conformational flexibility inherent in the identified ligands. I...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20508
更新日期:2006-01-01 00:00:00
abstract::H-Pro-Pro-Asp-NH2 is a highly active and selective catalyst for asymmetric aldol reactions. Here, the versatility of H-Pro-Pro-Asp-NH2 has been further improved by immobilization on a solid support and functionalization with a short polyethylene glycol linker at the C-terminus. The development, synthesis, and the cata...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20393
更新日期:2006-01-01 00:00:00
abstract::Combinatorial chemistry has recently burst on the scene as a valuable tool for the discovery of new drug candidates. The ability to synthesize hundreds of compounds for screening is a useful complement to rational drug design. There are many similarities between the design of new therapeutic agents and the development...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20395
更新日期:2006-01-01 00:00:00
abstract::Relevant parameters and stereochemical consequences of helices [alpha-helix, 3(10)-helix, beta-bend ribbon spiral, gamma-helix, 2.0(5)-helix, poly(Pro)(n) type-I and -II helices, and collagen triple helix] of peptides based on alpha-amino acids for use as templates in various branches of chemistry are briefly discusse...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20357
更新日期:2006-01-01 00:00:00
abstract::In order to check our current knowledge on the principles involved in beta-hairpin formation, we have modified the sequence of a 3:5 beta-hairpin forming peptide with two different purposes, first to increase the stability of the formed 3:5 beta-hairpin, and second to convert the 3:5 beta-hairpin into a 2:2 beta-hairp...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20345
更新日期:2005-10-15 00:00:00
abstract::MD simulations have been carried out to understand the dynamical behavior of the DNA substrate of the Thermus aquaticus DNA methyltransferase (M.TaqI) in the methylation process at N6 of adenine. As starting structures, an x-ray structure of M.TaqI in complex with DNA and cofactor analogue (PDB code: 1G 38) and free d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20341
更新日期:2005-10-15 00:00:00
abstract::The open reading frame 4 (ORF 4) gene product of barley yellow dwarf virus (BYDV) may act as a movement protein (MP) by assisting the transport of viral genomic RNA across the nuclear envelope (NE) of host plant cells. To investigate interactions between BYDV MP and the NE, wild-type and mutant open reading frame (ORF...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20334
更新日期:2005-10-05 00:00:00
abstract::Structural formation of segments plays pivotal roles in protein folding and stability, but how the segment influences the structural ensemble remains elusive. We engineered two hybrid proteins by replacing the central helical segment of immunoglobulin G binding domain of streptococcal protein G with an alpha-helix or ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20283
更新日期:2005-09-01 00:00:00
abstract::Lung cancer is usually fatal once it becomes metastatic. However, in order to develop metastases, a tumor usually invades the basal membrane and enters the vascular or lymphatic system. In this study, a three-dimensional artificial membrane using collagen type I, one of the main components of basal membranes, was esta...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20297
更新日期:2005-08-15 00:00:00
abstract::The degradation of elastin, the insoluble biopolymer of tropoelastin, can lead to the production of small peptides. These elastin-derived peptides (EDPs) are playing a key role in cellular behavior within the extracellular matrix, showing a great variety of biological effects such as chemotaxis, stimulation of cell pr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20276
更新日期:2005-07-01 00:00:00
abstract::The physicochemical characteristics of the green fluorescent protein (GFP), including the thermodynamic properties (entropy, enthalpy, Gibbs' free energy, heat capacity), normal mode vibrations, and atomic fluctuations, were investigated. The Gaussian 03 computational chemistry program was employed for normal mode ana...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20264
更新日期:2005-06-15 00:00:00
abstract::Thermal unfolding of bovine serum albumin (BSA) has been studied in the presence of 2,2,2-trifluoroethanol (TFE) using high-sensitivity microdifferential scanning calorimetry. Quantitative thermodynamic parameters accompanying the thermal transitions have been evaluated. TFE is observed to be a stabilizer or a destabi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20262
更新日期:2005-06-05 00:00:00
abstract::DMT1, also known as Nramp2, is an iron transporter, and belongs to the family of Nramp proteins. Disease-causing mutations both in Nramp1 and Nramp2 occurring at the conserved two adjacent glycine residues located within the fourth transmembrane domain (TM4) suggest that TM4 may serve an important biological function....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20204
更新日期:2005-03-01 00:00:00
abstract::Tropoelastin is a complex polymeric protein composed primarily of repeating segments of Val-Pro-Gly-Gly, Val-Pro-Gly-Val-Gly, and Ala-Pro-Gly-Val-Gly-Val that occurs in connective tissue and arteries. It has rubber-like extensible properties. A synthetic cyclic dodecapeptide, with a double repeat of the hexapeptide se...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20214
更新日期:2005-03-01 00:00:00
abstract::MUC1 mucin is a large transmembrane glycoprotein whose extracelluler domain is composed of repeating units of a 20 amino acid sequence. In the cancer associated state, this protein expression becomes upregulated and underglycosylated. Previous studies, which show an enhanced binding of a 5-repeat over a 1-repeat MUC1 ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20190
更新日期:2005-02-05 00:00:00
abstract::Small disulfide-rich peptides are translated as larger precursors typically containing an N-terminal prepro sequence. In this study, we investigated the role of a propeptide in the oxidative folding of an extremely hydrophobic delta-conotoxin, PVIA. delta-Conotoxin PVIA (delta-PVIA) is a 29-amino acid neurotoxin stabi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20211
更新日期:2005-01-01 00:00:00
abstract::The large abundance of bioactive single- and multiple-stranded cystine-rich peptides in nature has fostered the development of orthogonal thiol-protection schemes and of efficient chemistries for regioselective disulfide formation in synthetic replica for decades. In parallel to these entirely synthetic strategies, an...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20174
更新日期:2005-01-01 00:00:00
abstract::We studied fluorescence enhancements of fluorescein tethered to silver colloids of different size. Thiolated 23-mer oligonucleotide (ss DNA-SH) was bound selectively to silver colloids deposited on 3-aminopropyltriethoxysilane (APS)-treated quartz slides. Fluorescein-labeled complementary oligonucleotide (ss Fl-DNA) w...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20179
更新日期:2005-01-01 00:00:00
abstract::Rules relating the stereochemistry of N-Dnp (Dnp: 2,4-dinitrophenyl) derivatives of alpha-amino acids and peptides and the sign of the Cotton effects at the longest wavelength band (ca. 400 nm) are surveyed. Some new data and insights concerning the CD spectra of Dnp-alpha-amino acids are included: i.e., the spectra o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20198
更新日期:2005-01-01 00:00:00
abstract::Dermaseptins, small polycationic peptides synthesized by amphibians, exert a lytic action on bacteria, protozoa, yeast, and filamentous fungi at micromolar concentrations, but unlike polylysines, show little hemolytic activity. Dermaseptins S are active only against bacteria and form aggregates at high peptide/lipid r...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20244
更新日期:2005-01-01 00:00:00
abstract::We developed dictionaries of two-, three-, and five-residue patterns in proteins and computed the average solvent accessibility of the central residues in their native proteins. These dictionaries serve as a look-up table for making subsequent predictions of solvent accessibility of amino acid residues. We find that p...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20113
更新日期:2004-10-15 00:00:00
abstract::We describe here a new method for determining the conformational stability of antiparallel beta-sheets. Due to coupling between the transition dipoles, beta-sheet conformations typically exhibit a characteristic high-frequency amide I component centered at approximately 1680 cm(-1). Using one beta-sheet protein and tw...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20101
更新日期:2004-10-05 00:00:00
abstract::Vibrational absorption and vibrational circular dichroism (VCD) spectra of valinomycin are measured, in different solvents, in the ester and amide carbonyl stretching regions. The influence of cations, namely Li(+), Na(+), K(+), and Cs(+), in methanol-d(4) solvent is also investigated. Ab initio quantum mechanical cal...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20103
更新日期:2004-09-01 00:00:00
abstract::Fluorescein-labeled antibodies are widely used in clinical assays and fluorescence microscopy. The fluorescent signal per labeled antibody is limited by fluorescein self-quenching, which occurs when the antibody is heavily labeled with multiple fluoresceins. We examined immunoglobulin G (IgG) when labeled with 0.7 to ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20098
更新日期:2004-08-15 00:00:00
abstract::Reactive oxygen species (ROS) have generated a great deal of interest in the clinical field since experimental studies showed the involvement of these species in carcinogenesis. This paper reports the detection of ROS during the decomposition of H2O2 in the presence of samples obtained from tissues of 16 patients with...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20068
更新日期:2004-08-05 00:00:00
abstract::The structural evolution of regenerated Bombyx mori silk fibroin during shearing with a Couette cell has been studied in situ by synchrotron radiation small- and wide-angle x-ray scattering techniques. An elongation of fibroin molecules was observed with increasing shear rate, followed by an aggregation phase. The agg...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20083
更新日期:2004-07-01 00:00:00
abstract::Poly(dimer acid-brassylic acid) [P(DA-BA)] copolymers and poly(dimer acid-pentadecandioic acid) [P(DA-PA)] copolymers were prepared by melt polycondensation of the corresponding mixed anhydride prepolymers. The copolymers were characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), dif...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20078
更新日期:2004-06-15 00:00:00
abstract::It is shown by using the vibronic approach that the iron displacement out of the porphyrin plane in deoxyheme proteins intermixes the porphyrin pi and axial iron-histidine sigma electronic subsystems. This intermixing explains the substantial coupling of the iron-histidine vibration to the heme Soret excitation, the a...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20039
更新日期:2004-05-01 00:00:00
abstract::The folding of membrane proteins was addressed using outer membrane protein porin from the soil bacterium Paracoccus denitrificans (P. den.). IR spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis were used to probe the effect of mutagenesis on the thermal stability of the pr...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20049
更新日期:2004-05-01 00:00:00
abstract::Using a variety of spectroscopic techniques, a number of molecular functionalities have been studied in relation to the adsorption process of proteins to air-water interfaces. While ellipsometry and drop tensiometry are used to derive information on adsorbed amount and exerted surface pressure, external reflection cir...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20036
更新日期:2004-05-01 00:00:00
abstract::Water-mediated contacts are known as an important recognition tool in trp-repressor operator systems. One of these contacts involves two conserved base pairs (G(6).C(-6) and A(5). T(-5)) and three amino acids (Lys 72, Ile 79, and Ala 80). To investigate the nature of these contacts, we analyzed the X-ray structure (PD...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20023
更新日期:2004-04-15 00:00:00
abstract::Targeting double-stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA-PNA chimeras. A 16-mer 5'-DNA-3'-p-(N)PNA(C) has been investigated for its ability to hybridize a complementary d...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10599
更新日期:2004-03-01 00:00:00
abstract::Spray drying is a way to generate protein solids (powders), which is also true for lyophilization. Sugars are used to protect proteins from conformational changes and chemical degradations arising from drying processes and storage conditions such as the humidity. The influence of trehalose and humidity on the conforma...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10558
更新日期:2004-03-01 00:00:00
abstract::The various motifs of RNA molecules are closely related to their structural and functional properties. To better understand the nature and distributions of such structural motifs (i.e., paired and unpaired bases in stems, junctions, hairpin loops, bulges, and internal loops) and uncover characteristic features, we ana...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10525
更新日期:2004-02-15 00:00:00
abstract::Alternating sequences of D and L residues in peptides are directly related to the formation of several kinds of regular helical conformations usually called beta-helices. The major feature of these structures is that they can be associated with the transmembrane ion-conducting channel activity in some natural antibact...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10549
更新日期:2004-02-05 00:00:00
abstract::A novel nucleic acid model using peptide ribonucleic acid (PRNA), which contains 5'-amino-5'-deoxypyrimidine ribonucleoside as a recognition site for nucleic acids, has been designed, synthesized and applied to the external reversible control of recognition behavior of the complementary polynucleotide through the orie...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10563
更新日期:2004-01-01 00:00:00
abstract::The total synthesis of a peptide segment corresponding to the DNA-binding segment of Sp1 (positions 532-623) using a native chemical ligation approach is described. The folding of the synthetic segment in the presence of Zn(II) gave a zinc-coordinated protein. The dissociation constant (K(D)) for the DNA binding of th...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10562
更新日期:2004-01-01 00:00:00
abstract::Second-generation dendrimers have been prepared on solid phase by successive additions of branched polyproline building blocks starting from two different branching units anchored to the solid support. The preparation of Pro-rich building blocks was carried out by stepwise solid-phase synthesis and their iterative add...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20125
更新日期:2004-01-01 00:00:00
abstract::Proteins and nucleic acids are able to adopt their native conformation and perform their biological role only in the presence of water with which they actively interact in a mutually modifying way. Traditionally, hydrophobic effect has been considered to be the major factor stabilizing biopolymeric structures. However...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.10538
更新日期:2003-12-01 00:00:00